455 Characterizing skin aging using two-photon fluorescence and second harmonic generation
نویسندگان
چکیده
منابع مشابه
Two Photon Microscopy and Second Harmonic Generation
Nonlinear microscopy has become an important tool for imaging biological samples such as neurons. In two photon fluorescence, a fluorophore absorbs two photons at a low energy state, one of its electrons briefly converts to a higher energy state, and it emits a single excited photon at an energy level higher than that of the absorbed photons. In second harmonic generation (SHG), laser light is ...
متن کاملSynthesis of chromophores combining second harmonic generation and two photon induced fluorescence properties.
The design of new chromophores presenting simultaneous SHG and TPEF properties is reported.
متن کاملMembrane potential detection with second-harmonic generation and two-photon excited fluorescence: A theoretical comparison
We theoretically compare the performance of TPEF and SHG microscopy for membrane potential imaging. We argue that electrochromic TPEF and SHG membrane potential responses are reflections of the same phenomenon, and can be described in a unified manner as resulting from the linear Stark effect. We also show that TPEF and SHG exhibit similar sensitivities in the case of both electrochromic and or...
متن کاملMyofibrillogenesis in live neonatal cardiomyocytes observed with hybrid two-photon excitation fluorescence-second harmonic generation microscopy.
We developed a hybrid two-photon excitation fluorescence-second harmonic generation (TPEF-SHG) imaging system with an on-stage incubator for long-term live-cell imaging. Using the imaging system, we observed the addition of new sarcomeres during myofibrillogenesis while a cardiomyocyte was spreading on the substrate. The results suggest that the TPEF-SHG imaging system with an on-stage incubato...
متن کاملTwo-photon resonant second harmonic generation in atomic xeon.
Vacuum ultraviolet (VUV) radiation at 124.8 nm (9.93 eV) was produced from two-photon resonant second harmonic generation (SHG) in a xeon gaseous medium and used to probe molecular samples of acetone, furan, thiophene, ammonia, and methane. The mass spectra recorded from the species with ionization energies below 9.93 eV were dominated by the parent ions. The parent ions were only observed when...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 2017
ISSN: 0022-202X
DOI: 10.1016/j.jid.2017.02.474